Technical Data
M4550
Monopolin (Kinetochore Associated Protein, Mam1)
Description:
A novel kinetochore associated protein, Mam1 (Monopolin), plays a key role in the mechanism of meiotic chromosome segregation. It controls kinetochore behavior in meiosis I and de nes a functional basis by which sister kinetochores are directed toward the same pole in meiosis I.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Monopolin protein from Saccharomyce cerevisiae.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide before the addition of 40% glycerol.
Specificity:
Reacts with the Monopolin protein from Saccharomyce cerevisiae.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Toth A, Rabitsch KP, Galova M, Schleiffer A, Buonomo SB, and Nasmyth K. Cell 2000 Dec 22;103(7):1155–68