		Technical Data
M4550-01A	Monopolin (Monopolin Complex Subunit MAM1, Monopolar Microtubule Attachment During Meiosis 1 Protein 1, YER106W)

Description:
A novel kinetochore associated protein, Mam1 (Monopolin), plays a key role in the mechanism of meiotic chromosome segregation. It controls kinetochore behavior in meiosis I and defines a functional basis by which sister kinetochores are directed toward the same pole in meiosis I.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Yeast	Rabbit

Concentration:

~1mg/ml

Immunogen:

Monopolin protein, expressed in Saccharomyce cerevisiae.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes S. cerevisiae Monopolin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Toth A, Rabitsch KP, Galova M, Schleiffer A, Buonomo SB, and Nasmyth K. Cell 2000 Dec 22;103(7):1155-68.