Technical Data
Media
Motility Indole Ornithine Medium (Powder)
M4678
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation (g/L):
Enzymatic Digest of Gelatin10
Enzymatic Diget of Casein10
YeastExtract3
Destrose1
Bromcresol Purple0.02
L-Ornithine5
Agar2
Total:31
Differentiation of enterobacteriaceae on basis of motility, indole production and ornithine decarboxylase activity.


Directions per Liter: Dissolve 31 grams per liter of distilled/deionized (DDI) water. Heat with stirring until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

pH (25C): 6.50.2

Microorganisms Tested: Enterobacter aerogenes ATCC 13048

Escherichia coli ATCC 25922: + Growth, + Motility, + Indole, + Ornithine

Klebsiella pneumoniae ATCC 13883: + Growth, - Motility, - Indole, - Ornithine

Proteus mirabilis ATCC 12453: + Growth, variable Motility, - Indole, + Ornithine


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.