Technical Data
Motility Test Agar (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/L
Agar 4
Beef Extract 3
Enzymatic Digest of Gelatin 10
Sodium Chloride 5
Detection of motility of gram negative enteric bacteria.

Appearance: Beige, homogeneous, free flowing powder

Solubility: Yellow-beige, trace to slightly hazy

pH (25C): 7.3 0.2

Expected Cultural Response:
Cultural response in Motility Test Agar incubated aerobically at 35 2C and examined for growth after 18-24 hours.

The organisms listed are the minimum that should be used for quality control testing.
Expected Results
Microorganism (CFU) Response Motility
E. coli ATCC 25922 Direct Inoculation Growth Positive
S. choleraesuis ATCC 13076 Direct Inoculation Growth Positive
S. aureus ATCC 25923 Direct Inoculation Growth Negative

Directions per Liter: Dissolve 22 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.