Technical Data
Mph1103I (AvaIII)
Molecular Biology Storage: -20CShipping: Blue Ice
5'-A T G C A^T-3'
3'-T^A C G T A-5'

Moraxella phenylpyruvica RFL1103

Concentration: 10u/ul

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.

R1625-04 (Included):
Restriction Enzyme Buffer E, 10X Incubate at 37C

R1625 (Included):
Restriction Enzyme Buffer A, 10X
Provided to for double digests.

Diluent Buffer:
10mM Tris-HCl, pH 7.4 at 25C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.

Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA,50% glycerol.

Enzyme Properties:
Stability during Prolonged Incubation: A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Methylation Effects:
Dam/Dcm/CpG/EcoKl: never overlaps- no effect
EcoBl: may overlap-effect not determined

Thermal Inactivation:
Enzyme is inactivated by incubation at 65C for 20min.

Compatible Ends: Alw21I, PstI, SdaI, SduI

Number of Recognition Sites in DNA:
Lambda: 14
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 1
pTZ19R/U: 0

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe
Quality Control:
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mph1103I.

Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Mph1103I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with10units of restriction endonuclease for 4 hours.

Blue/White Cloning Assay: pUC57 was digested at a unique site with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.