|Molecular Biology||Storage: -20°CShipping: Blue Ice|
5'-A T G C A^T-3'
3'-T^A C G T A-5'
Moraxella phenylpyruvica RFL1103
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer.
Restriction Enzyme Buffer E, 10X Incubate at 37°C
Restriction Enzyme Buffer A, 10X
Provided to for double digests.
10mM Tris-HCl, pH 7.4 at 25°C, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.
10mM Tris-HCl (pH 7.4 at 25°C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA,50% glycerol.
Stability during Prolonged Incubation: A minimum of 0.3units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Dam/Dcm/CpG/EcoKl: never overlaps- no effect
EcoBl: may overlap-effect not determined
Enzyme is inactivated by incubation at 65°C for 20min.
Compatible Ends: Alw21I, PstI, SdaI, SduI
Number of Recognition Sites in DNA:
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffe
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mph1103I.
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Mph1103I, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.
Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with10units of restriction endonuclease for 4 hours.
Blue/White Cloning Assay: pUC57 was digested at a unique site with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.