Technical Data
M4680
Mph1103I (AvaIII)
1000U
5000U
Molecular Biology Storage: -20CShipping: Blue Ice
Sequence:
5'-A T G C A^T-3'
3'-T^A C G T A-5'

Source:
Moraxella phenylpyruvica RFL1103

Concentration: 10u/ul

Unit Definition:
One unit is defined as the amount of Mph1103I required to digest 1ug of lambda DNA in 1 hour at 37C in 50ul of assay buffer.

Supplied with:
R1625: Restriction Enzyme Buffer A, 10X:
Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.

R1625-04: Restriction Enzyme Buffer, 10X:
Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl, pH 8.5, 10mM MgCl2, 100mM potassium chloride,, 0.1mg/ml BSA

Storage Buffer:
Supplied as a liquid in 10mM Tris-HCl (pH 7.4 at 25C), 200mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol.

Enzyme Properties:
Stability during Prolonged Incubation: A minimum of 0.3 units of Mph1103I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C.

Methylation Effects:
Dam/Dcm/CpG/EcoKl: never overlaps- no effect
EcoBl: may overlap-effect not determined

Thermal Inactivation:
Mph1103I is inactivated by incubation at 65C for 20min.

Compatible Ends:
Alw21I, PstI, SdaI, SduI

Number of Recognition Sites in DNA:
Lambda: 14
PhiX174: 0
M13mp18/19: 0
pBR322: 0
pUC18/19: 0
pUC57: 1
pTZ19R/U: 0

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quality Control:
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Mph1103I.

Ligation/Recutting Assay: The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100.

Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Mph1103I for 4 hours.

Blue/White Cloning Assay: The B/W assay was replaced with LO test after validating experiments showed LO test ability to detect nuclease and phosphatase activites with sensitivity that equals to that of B/W test.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.