Technical Data
M4685-83A
MPS1 (Metallopan-stimulin 1, Metallopanstimulin 1, MPS-1, 40S Ribosomal Protein S27, Ribosomal Protein S27, RPS27)
Description:
Target protein MPS1/RPS27, also known as ribosomal protein S27, RPS27, 40S ribosomal protein S27, metallopanstimulin 1, MPS1, and MPS-1, can catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rats indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Human MPS1/RPS27 has been reported to be 84 amino acids with an apparent molecular weight of 9.5kDa.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 0.1-0.5ug/ml
Western Blot: 1.0-2.0ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9E10Affinity Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide conjugated to KLH.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA and 0.02% sodium azide.
Specificity:
Recognizes endogenous level of human MPS1/RPS27. I
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.