		Technical Data
M4688-10Y	MRPL18 (Mitochondrial Ribosomal Protein L18, 39S Ribosomal Protein L18 Mitochondrial, MRP-L18, HSPC071, L18mt)

Description:
MRPL18 is encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the Center region of human MRPL18 (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human MRPL18.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Zhang,Z. and Gerstein,M. Genomics 81(5), 468-480 (2003). 2.Koc,E.C., Burkhart,W. J. Biol. Chem. 276(47), 43958-43969 (2001). 3.Kenmochi,N., Suzuki,T. Genomics 77(1-2), 65-70 (2001).