Technical Data
M4688-20D
MRP2 (Multidrug Resistance-associated Protein 2, ABC30, ATP-binding Cassette Sub-family C Member 2, ATP-binding Cassette Sub-family C (CFTR/MRP) Member 2, ABCC2, Canalicular Multidrug Resistance Protein, CMRP, Canalicular Multispecific Organic Anion Trans
Description:
Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry (acetone fixed): 1:20-50 dil. on acetone fixed cytospin preparations. For Immunohistochemistry (acetone fixed frozen): 1:20
Western Blot: 1:20-50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aM2III-6Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
~250ug Ig/ml.
Immunogen:
Bacterial fusion protein of cMOAB/MRP2, containing the 202-amino acid COOH terminal end of the protein.
Purity:
Supernatant
Form
Supplied as a liquid in 0.7% BSA, 0.1% sodium azide.
Specificity:
Recognizes M2III-6 with an internal epitope of cMOAT/MRP2, a 170-180kD transmembrane protein known as the canalicular multi-organic anion transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Paulusma et al, Congenital jaundice in rats with a mutation in a multidrug resistance associated Protein gene. Science 271, 1126-1128, 1996. 2. Kool et al., Analysis of expression of cMOAB (MRP2), MRP3, MRP4 and MRP5, homologs of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res 57, 3537-3547, 1997. 3. Scheffer et al, Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5 and MDR3 P-glycoprotein with a panel of monoclonal antibodies, Cancer Res. 60:5269-5277, 2000.