Technical Data
MRP6 (Multidrug Resistance-associated Protein 6, ABC34, ATP-binding Cassette Sub-family C Member 6, ABCC6, Anthracycline Resistance-associated Protein, ARA, EST349056, MLP1, Multi-specific Organic Anion Transporter E, MOATE, MOAT-E, PXE, PXE1, URG7)
Suitable for use in Immunocytochemistry Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 1:20-1:50 on acetone-fixed cytospin preparations.
Immunohistochemistry: 1:20-1:50 on acetone-fixed, frozen sections followed by incubation with IgG rabbit anti-rat and an APAAP complex. Note: This antibody is unreactive on standard formaldehyde-fixed, paraffin-embedded material.
Western Blot: 1:20-1:50. Use IgG (HRP) anti-rat as secondary antibody.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceHumanRat
~100ug IgG/ml
Bacterial fusion protein of human MRP6, containing aa764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein.
Culture supernatant
Supplied as a liquid containing 1% BSA and 0.09% sodium azide.
Recognizes human MRP6, a 190-200kD transmembrane protein that is related to the multidrug resistance related protein MRP. Does not cross-react with the human MDR1, MRP1, MRP2, MRP3, MRP4 and MRP5 gene products.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bergen AA, Plomp AS, Schuurman EJ, Terry S, Breuning M, Dauwerse H, Swart J, Kool M, van Soest S, Baas F, ten Brink JB, de Jong PT. Mutations in ABCC6 cause pseudoxanthoma elasticum. Nat Genet 2000 Jun;25(2):228-31 2. Scheffer GL, Hu X, Pijnenborg AC, Wijnholds J, Bergen AA, Scheper RJ. 3. MRP6 (ABCC6) detection in normal human tissues and tumors. 4. Lab Invest 2002 Apr;82(4):515-8.