Technical Data
M4692-36F
MSH6 (MutS Homolog 6 (E. coli), DNA Mismatch Repair Protein MSH6, G/T Mismatch Binding Protein, GTBP, GTMBP, Hereditary Nonpolyposis Colorectal Cancer Type 5, HNPCC5, MutS alpha 160kD Subunit, p160, Sperm Associated Protein, hSAP)
Description:
Defects in MSH6 are a cause of hereditary non-polyposis colorectal cancer (HNPCC) (Lynch syndrome). HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. it is characterized by a familial predisposition to early onset colorectal carcinoma (crc) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the western world. MSH6 is central to mismatch DNA repair. The MSH2-MSH6 complex recognizes a single-based mispair insertion/deletion loop. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related human carcinogenesis. Studies have shown the mutations of MLH-1, MSH2 and MSH6 genes contribute to the development of sporadic colorectal carcinoma. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): Neat
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil, colon carcinoma

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18L806Affinity Purified
SizeStorageShippingSourceHost
6ml4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to human MSH6. Cellular Localization: Nuclear.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, sodium azide, BSA, 6M Urea.
Specificity:
Recognizes human MSH6. MSH6 is a heterodimer of MSH2 and binds to DNA containing G/T mismatches. Species Crossreactivity: mouse, rat and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Frasno et al. Mod Pathol 11: 934, 1998. 2. Schleger et al. Exp Cell Res 236: 418, 1997.