Technical Data
M4692-44B
MSK1, phosphorylated (Thr581) (Mitogen and Stress Activated Protein Kinase 1, MSPK1, 90kD Ribosomal Protein S6 Kinase 5, Nuclear Mitogen And Stress Activated Protein Kinase 1, Ribosomal Protein S6 Kinase 90kD Polypeptide 5, Ribosomal Protein S6 Kinase Alp
Description:
MSK1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364, Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic phosphopeptide surrounding Thr581 from human MSK1.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
Specificity:
Recognizes human Phospho-MSK1 at ~90kD. Does not cross-react with nonphosphorylated MSK1, MSK1 phosphorylated at other sites, RSK1, RSK2 or RSK3. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.