Technical Data
M4693-15A
MS-275 (Entinostat, SNDX-275)
25mg
Biochemicals Storage: -20°CShipping: Blue Ice
MS-275 is a synthetic benzamide derivative that inhibited partially purified human histone deacetylase (HDA) with an IC50 of 2uM and induced hyperacetylation of nuclear histones in tumor cell lines. MS-275 inhibited the proliferation of various human tumor cell lines such as A2780, Calu-3, HL-60, and K562 (IC50 values were 0.0415uM, 0.195uM, 0.212uM, and 0.589uM, respectively). MS-275 also inhibited the growth of human tumor xenografts in nude mouse.

Solubility:
Soluble in DMSO at 25mg/ml with slight warming; very poorly soluble in ethanol and water; maximum solubility in plain water is estimated to be about 10-20uM. buffers, serum, or other additives may increase or decrease the aqueous solubility.

Melting Point:
216-218ºC

Elemental Anaylsis:
Calculated:
C=67.01%
H=5.36%
N=14.88%

Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

CAS Number:
209783-80-2

Molecular Formula:
C21H20N4O3

Molecular Weight:
376.41
Purity: ~99%
Form: Supplied as a white to off-white powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Saito, A., et al. "A synthetic inhibitor of histone deacetylase, MS-27-275, with marked in vivo antitumor activity against human tumors." Proc. Natl. Acad. Sci. USA 96:4592-4597 (1999). 2. Lee, B.I., et al. "MS-275, a histone deacetylase inhibitor, selectively induces transforming growth factor beta type II receptor expression in human breast cancer cells." Cancer Res. 61:931-934 (2001). 3. Eyüpoglu, I.Y., et al. "Experimental therapy of malignant gliomas using the inhibitor of histone deacetylase MS-275." Mol. Cancer Ther. 5:1248-1255 (2006). 4. Gojo, I., et al. "Phase 1 and pharmacologic study of MS-275, a histone deacetylase inhibitor, in adults with refractory and relapsed acute leukemias." Blood 109:2781-2790 (2007). 5. Lin, H.S., et al. "Anti-rheumatic activities of histone deacetylase (HDAC) inhibitors in vivo in collagen-induced arthritis in rodents." Br. J. Pharmacol. 150:862-872 (2007). 6. Khandelwal, A., et al. "MS-275 synergistically enhances the growth inhibitory effects of RAMBA VN/66-1 in hormone-insensitive PC-3 prostate cancer cells and tumours." Br. J. Cancer 98:1234-1243 (2008).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.