Technical Data
M4696-11
mTOR, phosphorylated (Ser2448) (mTAb1, Mammalian Target of Rapamycin, FRAP, RAFT)
Description:
Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12-rapamycin associated protein 1, FK506 binding protein12-rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12- rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:15,000-1:65,000
Western Blot: 1:500-1:2000
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide correspoding to aa2440-2457 of human mTOR.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes phosphorylated mTOR from human derived tissues and cells. Species sequence homology: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kristof,A.S. et al. (2003) J. Biol. Chem. 278 (36), 33637-33644. 2. Chen,Y., et al. (2003) . Oncogene 22 (25), 3937-3942. 3. Nojima,H. et al. (2003). J. Biol. Chem. 278 (18), 15461-15464. 4. Nojima,H. et al. (2003) J. Biol. Chem. 278 (18), 15461-15464. 5. McMahon,L.P. et al. (2002) . Mol. Cell. Biol. 22 (21), 7428-7438. 6. Hudson,C.C. et al. (2002) 6. Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin. Mol. Cell. Biol. 22 (20), 7004-7014. 7. Choi,J.H. et al. (2002). EMBO Rep. 3 (10), 988-994. 8. Inoki,K. et al. (2002) . Nat. Cell Biol. 4 (9), 648-657. 9. Zhang,X. et al. (2002) J. Biol. Chem. 277 (31), 28127-28134.