Description:
Mucin is an important tumor-associated marker for adenocarcinomas.
Applications:
Suitable for use in Immunofluorescence Microscopy and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Frozen and Paraffin): 1:10-1:40. Recommended dilution buffer is PBS, 1% BSA, 0.1% sodium azide.
Optimal dilutions to be determined by the researcher.
Positive Control:
Gastric mucosa of stomach
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 5F115 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Mucin isolated from ovarian mucinous cysts and colonic mucosa. Cellular Localization: cytoplasm |
| Purity: |
| Culture supernatant |
| Form |
| Supplied as a liquid, 0.09% sodium azide. |
| Specificity: |
| Recognizes Mucin. Shows specificity to gastric mucosa, but is also reactive with stomach and colon carcinomas. Most breast carcinomas are negative. Reactive with the g epitope on Mucin M1. Species Crossreactivity: Human, chicken, most mammals (including monkey, feline, porcine, rat, mouse, rabbit, hedgehog). Does not react with bovine. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Bara, J., et al., J. Immunol. Meth. 149: 105-113 (1992). 2. Bara, J., et al., Int. J. Cancer 47: 304-310 (1991).
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