Technical Data
M4700-05E
Mucin 1 (MUC1 Glycoprotein, MUC-1, Breast Carcinoma Associated Antigen DF3, CA 15-3, Carcinoma Associated Mucin, CD227, DF3, EMA, Episialin, Epithelial Basement Membrane Antigen, Epithelial Membrane Antigen, Epithelial Mucin Tandem Repeat Sequence, H23 An
Description:
Detection of un-glycosylated and underglycosylated Mucin-1 Glycoprotein

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Af nity Constant:
1.0 x 10e8L/mole

Cellular Localization:
Cell Membrane and Secreted

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG8L815Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
High molecular weight (>300 kD) glycoprotein from human milk-fat globule molecule. Epitope: Region of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes VNTR5 and VNTR20 recombinant unglycosylated fragments of human MUC1 protein and underglycosylated MUC1. Does not recognize natural MUC1 protein. Does not react with egg white avidin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.