Technical Data
M4700-07
Mucin 1 (MUC1 Glycoprotein, CD227)
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Technical Data |
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M4700-07 |
Mucin 1 (MUC1 Glycoprotein, CD227) |
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Description: Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: ELISA: Suitable for the detection of under-glycosylated tumor-associated MUC1 mucins in human serum. Immunohistochemistry (FFPE and Frozen): 50ug/ml or less Optimal dilutions to be determined by the researcher. Affinity Constant: >1x10e8 Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice Recommended ELISA pairs: Capture: M4700-08 or M4700-05 Detection: M4700-07 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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