Technical Data
M4700-07
Mucin 1 (MUC1 Glycoprotein, CD227)
Description:
Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: Suitable for the detection of under-glycosylated tumor-associated MUC1 mucins in human serum.
Immunohistochemistry (FFPE and Frozen): 50ug/ml or less
Optimal dilutions to be determined by the researcher.

Affinity Constant:
>1x10e8

Hybridoma:
X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice

Recommended ELISA pairs:
Capture: M4700-08 or M4700-05
Detection: M4700-07

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1.B.877Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
High molecular weight (>300kD) glycoprotein from human milk-fat globule molecule
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes underglycosylated and natural MUC1 protein (isolated from human milk by affinity chromatography). Does not crossreact with normal serum proteins and human tissues of nonepithelial origin. Binds with high efficiency with different epitopes within the VNTR tandem repeat peptide conformations and peptide- carbohydrate compositions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.