Technical Data
M4700-09B
Mucin 1 (MUC1 Glycoprotein, CD227)
Description:
Epithelial Membrane Antigen, also referred to as EMA, CA15-3, Polymorphic Epithelial Mucin, PEM, Sialomucin or Episialin and MUC-1 is a glycoprotein of variable molecular weight, expressed by many glandular epithelial cells. Epithelia of breast, (acini and ducts), exocrine pancreas, bladder, gastrointestinal tracts, thyroid, endometrium and respiratory tract are generally EMA positive.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Immunohistochemistry: 1:100. Anti-MUC-1 IgY staining of Formalin-fixed and paraffin-embedded Esophogeal Cancer tissue. Affinity-purified IgY dilution: 1:100, rabbit anti-IgY-biotin dilution: 1:300.
Optimal dilutions to be determined by the researcher.

Molecular Weight:
122.1kD (calculated)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanChicken
Concentration:
~1mg/ml
Immunogen:
Recombinant human protein Mucin 1, aa199-244. (NCBI Accession number: NP_001125.1, Swiss Prot Accession number: P02771, Immunogen Sequence: Gi # 65301117)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes human Mucin 1. May play a role in adhesive functions and in cell-cell interactions, matastasis, signaling, and direct/indirect interaction with actin cytoskeleton. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.