Technical Data
M4700-11C
Mucin 1 (MUC1 Glycoprotein, CD227)
Description:
Mucins are heavily glycosylated glycoproteins, which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes (Muc-1, 2, 3, 4, 5AC, 5B, 6, 7 and 8) have been identified. Various immunohistochemical and in situ hybridization studies have shown that these mucins are differentially expressed in epithelia with cell-type specificity. Muc-5AC and Muc-6 glycoproteins are the first two mucins found in superficial epithelium and Muc-6 glycoprotein is found in the deep glands. Belongs to monoclonal cluster 1-4 according to ISOMB TD-4 classification.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen tissue sections or formalin fixed paraffin wax embedded tissue sections.
Note: HIER (Microwave, citrate buffer pH 6.0) may be advantageous for some applications.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q441Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human breast cancer cell line ZR-75-1.
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 10mM sodium azide.
Specificity:
Recognizes the extracellular human 12-mer GVTSAPDTRPAP epitope of the MUC-1 tandem repeat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.