		Technical Data
M4700-89	Muc4 (Mucin-4, MUC-4, Ascites Sialoglycoprotein, ASGP, Pancreatic Adenocarcinoma Mucin, Pre-sialomucin Complex, pSMC, Sialomucin Complex, Testis Mucin, Smc)

Description:
MUC4 is a membrane-associated protein of the mucin (MUC) gene family, encoded by a gene on chromosome 3q29 and produced by epithelial cells as a heterodimer. The MUC4 protein is thought to play a protective role for vulnerable epithelia, particularly in the airway, eye, female reproductive tract and mammary gland. Two observations support this hypothesis: (a) MUC4 acts as an anti-adhesive barrier at epithelial and tumor surfaces, and (b) MUC4 binds the receptor tyrosine kinase ErbB2, and can alter its downstream signaling. Alterations in MUC4 expression have been observed in association with a variety of inflammatory and neoplastic states; reduction or loss has been reported in non-small cell lung carcinoma, hyperplastic polyps of the colon, and serrated colon adenomas, while overexpression of the MUC4/Sialomucin complex (SMC) has been identified in malignant progression of mammary tumors in both humans and rats.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin fixed, paraffin-embedded tissue sections; Epitope retrieval is required using EDTA, pH 8.0. See Protocol.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Colon

Expected Staining Pattern:
Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6A134 (1G8)	Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Rat	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Rat ASGP-2

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.

Specificity:

Recognizes rat MUC4. In normal tissues, stains stomach, colon, and the endothelial cells of small blood vessels and capillaries. In abnormal tissues, M4700-89 exhibits strong positive staining in colon polyps (3/3 specimens tested), colon carcinoma (4/4), and gastric adenocarcinoma (4/5). Lung adenocarcinoma and ovarian mucinous adenocarcinoma also demonstrated positive staining. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Perrais, M., et al., J. Biol. Chem. 276 (33): 30,923-30,933 (2001). 2. Carraway, K.L., et al., J. Mammary Gland Biol. Neoplasia 6(3): 323-337 (2001). 3. Lopez-Ferrer, et al., Am. J. Respir. Cell Mol. Biol. 24(1): 22-29 (2001). 4. Biemer-Huttmann, A.E., et al., J. Histochem. Cytochem. 47(8): 1039-1047 (1999). 5. Komatsu, M., et al., Oncogene 20(4): 461-470 (2001).