Technical Data
M4701-85D
MUC13 (Mucin-13, MUC-13, Down-regulated in Colon Cancer 1, DRCC1, RECC, UNQ6194/PRO20221)
Description:
Mucin 13 is a 512aa hemopoietic transmembrane mucin belonging to a family of secreted and cell surface glycoproteins and may play a role in cell signaling. It contains a serine/threonine-rich mucin repeat domain predicted to be heavily O-glycosylated, three cysteine-rich EGF-like domains, a SEA domain and also has sites for N-glycosylation and phosphorylation. Activation of NF-kappa-B signaling pathway by the inflammatory cytokines TNF-alpha and TGF-beta leads to upregulation of Mucin 13. It is a homodimer of beta subunits and is highly expressed in epithelial tissues, gastrointestinal and respiratory tracts, kidney, small intestine, appendix and stomach. RT-PCR and immunoblot analysis supports the up regulation of Mucin 13 in gastric cancers and is usually present on the apical side of tubular glands in intestinal-type cancers and in the cytoplasm in diffuse cancers.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa480-530 of human Mucin 13.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Mucin 13. Species sequence homology: chimpanzee, monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moehle, C. et al. J. Molec. Med. 84:1055-1066 (2006). 2. Shimamura, T. et al. Cancer Sci. 96:265-273 (2005). 3. Williams, SJ. et al. J. Biol. Chem. 276:18327-18336 (2001).