		Technical Data
M4701-85G	MUC13 (Mucin-13, MUC-13, Down-regulated in Colon Cancer 1, DRCC1, RECC, UNQ6194/PRO20221)

Description:
Mucin 13 is a 512aa hemopoietic transmembrane mucin belonging to a family of secreted and cell surface glycoproteins and may play a role in cell signaling. It contains a serine/threonine-rich mucin repeat domain predicted to be heavily O-glycosylated, three cysteine-rich EGF-like domains, a SEA domain and also has sites for N-glycosylation and phosphorylation. Activation of NF-kappa-B signaling pathway by the inflammatory cytokines TNF-alpha and TGF-beta leads to upregulation of Mucin 13. It is a homodimer of beta subunits and is highly expressed in epithelial tissues, gastrointestinal and respiratory tracts, kidney, small intestine, appendix and stomach. RT-PCR and immunoblot analysis supports the up regulation of Mucin 13 in gastric cancers and is usually present on the apical side of tubular glands in intestinal-type cancers and in the cytoplasm in diffuse cancers.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to DCKSDLQRPNPQ, from MUC13, at the internal region of the protein (NP_149038.2).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Specificity:

Recognizes MUC13. Species sequence homology: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Walsh MD, Young JP, Leggett BA, Williams SH, Jass JR, McGuckin MA. The MUC13 cell surface mucin is highly expressed by human colorectal carcinomas. Hum Pathol. 2007 Jun;38(6):883-92. Epub 2007 Mar 13.