Technical Data
Media
Mueller Hinton Medium (Agar) (Powder)
M5000
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation for 1 Liter:
Components shown as g/liter
Beef Extract.2
Acid Hydrolysate of Casein.17.5 Starch
Agar17
Total:38.0g/L
Mueller Hinton Agar is used in antimicrobial susceptibility testing by the disk diffusion method. This formula
conforms to National Committee for Clinical Laboratory Standards (NCCLS).

Quality Control:
Tested according to USB Standard Test Methods and NCCLS M6-A. Testing was performed in compliance with ISO/TS 11133-1 and BS EN 12322:1999 + A1:2001 standards. This product met or exceeded established NCCLS performance specifications.

Appearance:
Beige, homogenous,
free-flowing powder

Solubility:
Light to medium yellow, hazy, clear and complete with boiling.

pH:
7.3 0.1


Directions per Liter: Dissolve 38grams in 1L of ddH2O stirring gently with heating until completely solubilized. Boil for 1 minute. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

OPTIONAL: Supplement as appropriate. Pour cooled Mueller Hinton Agar into sterile petri dishes. Allow to cool to room temperature. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

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Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. National Committee for Clinical Laboratory Standards. 1997. Performance standards for antimicrobial disk susceptibility tests. Approved standard M2-A6. National Committee for Clinical Laboratory Standards, Wayne, PA. 2. Mueller, J. H., and J. Hinton. 1941. A protein-free medium for primary isolation of gonococcus and meningococcus. Proc. Soc. Exp. Biol. Med. 48:3330-333. 3. Gordon and Hine. 1916. Br. Med. J. 678. 4. Bauer, A. L., W. M. M. Kirby, J. C. Sherris, and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45:493-496. 5. World Health Organization. 1961. Standardization of methods for conducting microbic sensitivity tests. Technical Report Series No. 210, Geneva. 6. Food and Drug Administration. Bacteriological analytical manual, 8th ed., AOAC International, Gaithersburg, MD. 7. Wood, G. L., and J. A. Washington. 1995. Antibacterial susceptibility tests: dilution and disk diffusion methods, p. 1327-1341. In Murray, P.R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C. 8. National Committee for Clinical Laboratory Standards. 1996. Protocols for evaluating dehydrated; App. Standard. Wayne PA. 9. National Committee for Clinical Laboratory Standards. 1999. M100-S9. Performance Standards for Antimicrobial Susceptibility Testing; Ninth Informational Supplement. Wayne, PA. 10. Isenberg, H. D. (ed.). 1992. Clinical microbiology procedures handbook, vol. 1, American Society for Microbiology, Washington, D.C.