Technical Data
M9218
Mung Bean Nuclease
2000U
Molecular Biology Storage: -20°CShipping: Blue Ice
Mung Bean Nuclease belongs to a class of enzymes that demonstrate a preference for single-stranded nucleic acids, lack sugar specificity and hydrolyze single-stranded substrates to products with 5’-phosphoryl and 3’-hydroxyl termini. Mung Bean Nuclease catalyzes the degradation of single-stranded DNA and RNA endonucleolytically to yield 5'-phosphoryl terminated products. While the nuclease prefers ssDNA over dsDNA by 30,000-fold, at very high concentrations, such as 14u/ug of DNA, the enzyme degrades double-stranded DNA preferentially from both ends (1,2,3).

Unit Definition: One unit is defined as the amount of enzyme required to produce 1ug of acid-soluble nucleotides per minute at 37°C in 30mM sodium acetate, pH 5.0, 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA, 5% glycerol.

Additional Information
Molecular Weight: 39kD
Requirement: Zn2+.
Inhibitors: Mung Bean Nuclease is inhibited by high salt concentrations (80-90% inhibition in 200-400mM NaCl). Use with 0.001% Triton® X-100 when using very low concentrations (< 50u/ml), because under such conditions Mung Bean Nuclease may adhere to surfaces and is rather unstable (7).

Quality Control:
Contaminant Endonuclease/Nickase Activity: To confirm the absence of contaminating endonuclease/nickase activity, 1ug of lambda DNA/ Hind III markers is incubated with Mung Bean Nuclease for 10 minutes at RT. The markers are then separated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Markers that have been incubated with 60u of enzyme will remain as intact bands with a minimal amount of smearing.

Applications:
Suitable for use in Transcript Mapping, Production of blunt ends for cloning, Flushing staggered ends and Separation of cDNA strands after synthesis with reverse transcriptase and DNA polymerase I.

Reaction Buffer:
M9218A: Mung Bean Nuclease, Reaction Buffer (10X), 1x1ml
Form: Supplied as a liquid in 0.3Msodium acetate, pH 5.0, 0.5M sodium chloride, 10mM ZnCl2.
Recommended Dilution: 1:10

Storage and Stability:
Aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
39kD
Source: Mung bean sprouts (Phaseolus aureus)
Concentration: ~100u/ul
Form: Supplied as a liquid in 0.01M Tris-HCl, pH 7.5, 0.05M sodium chloride, 50% glycerol, 0.01% Triton X-100.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ardelt, W., Laskowski, M. Sr.: Mung bean nuclease I. IV. An improved method of preparation. Biochem. Biophys. Res. Comm. 44: 1205 (1971). 2. Kroeker, W.D., Kowalski, D., Laskowski, M. Sr.: Biochemistry15: 4463 (1976). 3. Kroeker, W., Spurgeon, S.: Mung bean nuclease (1985). 4. Mathis, D.J., et al.: Specific in vitroinitiation of transcription on the adenovirus type 2 early and late EII transcription units. PNAS USA 78: 7383 (1981). 5. Green, M.R., Roeder, R.G.: Definition of a novel promoter for the major adenovirus-associated virus mRNA. Cell 22: 231 (1980). 6. Gubler, U.: Second-strand cDNA synthesis: mRNA fragments as primers. Meth. Enzymol. 152: 330 (1987). 7. Perbal, B.: A Practical Guide to Molecular Cloning, 2nd ed., John Wiley and Sons, New York (1988).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.