Suitable for use in Westen Blot. Other applications not tested.
Western Blot: 1:200 Rat brain membranes
Optimal dilutions to be determined by the researcher.
Positive Control Antigen:
Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|50ul||4°C (-20°C Glycerol)||Blue Ice||Human||Rabbit|
|Fusion protein, GSETP GKGGGSSSSS ERSQP GAEGS PETPP GRCCR CCRAP RLLQAYSWKE EEEED EGSME SLTSS EGEEP GSEVV IKMPM VDPEA QAPTK QPPRS SPNTV KRPTK KGRDR AGKGQ KPRGK EQLAK RK, corresponding to aa227-353 of human M1 (Accession P11229). Epitope location: 3rd intra|
|Purified by immunoaffinity chromatography.|
|Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile, distilled, deionized water or sterile 40-50% glycerol.|
|Recognizes human Muscarinic Receptor M1. Species Crossreactivity: Rat. Species Sequence Homology: Macaca mulatta-125/127, mouse and porcine-124/127, rat and gebril-123/127.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|