Technical Data
M9520-01
Muscarinic Receptor M1, aa227-353 (Cholinergic Muscarinic Receptor M1, Chrm1, Muscarinic Receptor M1)
Description:
Applications:
Suitable for use in Westen Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200 Rat brain membranes
Optimal dilutions to be determined by the researcher.

Positive Control Antigen:
M9520-01A

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.8mg/ml
Immunogen:
Fusion protein, GSETP GKGGGSSSSS ERSQP GAEGS PETPP GRCCR CCRAP RLLQAYSWKE EEEED EGSME SLTSS EGEEP GSEVV IKMPM VDPEA QAPTK QPPRS SPNTV KRPTK KGRDR AGKGQ KPRGK EQLAK RK, corresponding to aa227-353 of human M1 (Accession P11229). Epitope location: 3rd intra
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Reconstitute with 50ul sterile, distilled, deionized water or sterile 40-50% glycerol.
Specificity:
Recognizes human Muscarinic Receptor M1. Species Crossreactivity: Rat. Species Sequence Homology: Macaca mulatta-125/127, mouse and porcine-124/127, rat and gebril-123/127.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.