Technical Data
M9520-03B
Muscarinic Receptor M1, aa451-460 (Cholinergic Muscarinic Receptor M1, Chrm1)
Description:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13.

Applications:
Suitable for use in Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:800
Immunocytochemistry: 1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not determined
Immunogen:
A peptide analogue of the carboxyl terminal of the Muscarinic M1 receptor covalently attached to a carrier protein.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity:
Recognizes muscarinic M1 receptor (carboxyl terminal) (aa451-460). Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.