The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).
Suitable for use in ELISA. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Recombinant protein ESAT6 M. tuberculosis H37RV|
|Purified by Protein G chromatography. >95% pure.|
|Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.|
|Recognizes the ESAT antigen of Mycobacterium tuberculosis.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Sorensen AL, et al., (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-17. 2. Ravn P,et al., (1999) Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645. 3. Klausen et al., (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-9. 4. Harboe M, et al., (1998) B-Cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-23. 5. Harboe M, et al., (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.|