Technical Data
M9699-58C
Mycobacterium tuberculosis ESAT6 (6kD early secretory antigen of T cells)
Description:
The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19B88Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to ESAT6 M. tuberculosis H37RV
Purity:
Purified by Protein G chromatography. >95% pure.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the ESAT6 antigen of Mycobacterium tuberculosis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorensen AL, et al., (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-17. 2. Ravn P,et al., (1999) Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645. 3. Klausen et al., (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-9. 4. Harboe M, et al., (1998) B-Cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-23. 5. Harboe M, et al., (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.