Technical Data
M9699-59
Mycobacterium tuberculosis
Description:
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31kD, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:200.
Optimal dilutions to be determined by the researcher.

Culture Medium:
Dulbeccos modified Eagles medium with 10% fetal calf serum.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6F152Affinity Purified
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Bacterial press extract from Mycobacterium tuberculosis H37Rv.
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein. No crossreactivity to antigens from Listeria monocytogenes or Escherichia coli. Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid). Reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1). In Western blotting after SDS-PAGE reacts strongly with Ag85C and not as strongly with Ag85A and Ag85B.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schou C, Yuan ZL, Andersen AB, Bennedsen J (1985) Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis. Acta Pathol Microbiol Immunol Scand (C) 93:265-272. 2. Andersen AB, Yuan ZL, Haslov K, Vergmann B, Bennedsen J (1986) Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol 23:446-451. 3. Worsaae A, Ljungqvist L, Heron I (1988) Monoclonal antibodies produced in BALB.B10 mice define new antigenic determinants in culture filtrate preparations of Mycobacterium tuberculosis. J Clin Microbiol 26:2608-2614. 4. Belisle JT, Vissa VD, Sievert T, Takayama K, Brennan PJ, Besra GS (1997) Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis. Science 276:1420-22. 5. D'Souza S, Rosseels V, Romano M, Tanghe A, Denis O, Jurion F, Castiglione N, Vanonckelen A, Palfliet K, Huygen K (2003) Mapping of murine Th1 helper T-Cell epitopes of mycolyl transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis. Infect Immun 71:483-493.