Technical Data
M9699-59F
Mycobacterium tuberculosis 16kD (M. tuberculosis)
Description:
Mycobacterium tuberculosis is a fairly large non-motile rod-shaped bacterium distantly related to the Actinomycetes. Many non pathogenic mycobacteria are components of the normal flora of humans, found most often in dry and oily locales. The rods are 2-4um in length and 0.2-0.5um in width. Mycobacterium tuberculosis is an obligate aerobe. For this reason, in the classic case of tuberculosis, the M.TB. complexes are always found in the well-aerated upper lobes of the lungs. The bacterium is a facultative intracellular parasite, usually of macrophages, and has a slow generation time, 15-20 hours, a physiological characteristic that may contribute to its virulence. M.TB. is not classified as either Gram-positiveor Gram-negative because it does not have the chemical characteristics of either, although the bacteria do contain peptidoglycan (murein) in their cell wall. If a Gram stain is performed on M.TB., it stains very weakly Gram-positive or not at all (referred to as "ghosts"). Mycobacterium species, along with members of a related genus Nocardia , are classified as acid-fast bacteria due to their impermeability by certain dyes and stains. Despite this, once stained, acid-fast bacteria will retain dyes when heated and treated with acidified organic compounds. One acid-fast staining method for Mycobacterium tuberculosis is the Ziehl-Neelsen stain . When this method is used, the M.TB. smear is fixed, stained with carbol-fuchsin (a pink dye), and decolorized with acid-alcohol. The smear is counterstained with methylene-blue or certain other dyes. Acid-fast bacilli appear pink in a contrasting background.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a9L483Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Purified protein derivative (PPD)
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.01M PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the 16kD antigen (HspX, alpha-crystallin) of M. tuberculosis and M. bovis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.