Technical Data
M9699-61
Mycobacterium tuberculosis (HRP)
Description:
Mycobacterium tuberculosis is the etiologic agent of tuberculosis (TB) in humans. Humans are the only reservoir for the bacterium. Mycobacterium bovis is the etiologic agent of TB in cows and rarely in humans. Both cows and humans can serve as reservoirs. Humans can also be infected by the consumption of unpasteurized milk. This route of transmission can lead to the development of extrapulmonary TB, exemplified in history by bone infections that led to hunched backs. Other human pathogens belonging to the Mycobacterium genus include Mycobacterium avium which causes a TB-like disease especially prevalent in AIDS patients, and Mycobacterium leprae, the causative agent of leprosy. Mycobacterium tuberculosis is a fairly large non-motile rod-shaped bacterium distantly related to the Actinomycetes. Many non pathogenic mycobacteria are components of the normal flora of humans, found most often in dry and oily locales. The rods are 2-4um in length and 0.2-0.5um in width. Mycobacterium tuberculosis is an obligate aerobe. For this reason, in the classic case of tuberculosis, the M.TB. complexes are always found in the well-aerated upper lobes of the lungs.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Molar HRP: IgG substitution: ~2-3

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
~1-2mg/ml
Immunogen:
Purified PPD. PPD is generated by boiling a culture of Mycobacterium tuberculosis, specifically Old Tuberculin (OT).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 10mg/ml BSA, 0.002% Thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes a minimum of 2 major Mycobacterium tuberculosis bands (gamma & beta) by Immunoelectrophoresis. Has not been absorbed and may crossreact with related microorganisms. Crossreacts with Rhodacoccus sp. Crossreactive with other Mycobacterial species including M. avium, M. phlei and M. parafortuitum. Does not crossreact with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus Group B, Candida albicans and Neisseria meningitidis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.