Description:
The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:3160
Optimal dilutions. to be determined by the researcher.
Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p.
Culture Medium:
RPMI 1640 with 10% fetal calf serum.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1,k | 6F153 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ul | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~0.5mg/ml |
| Immunogen: |
| PPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut) (1). |
| Purity: |
| Purified by Protein A/G affinity chromatography from culture supernatant. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide before the addition of glycerol to 40%. |
| Specificity: |
| Recognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 (4). Species Crossreactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Sorensen AL, et al., (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-17. 2. Ravn P,et al., (1999) Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645. 3. Klausen et al., (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-9. 4. Harboe M, et al., (1998) B-Cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-23. 5. Harboe M, et al., (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.
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