Technical Data
M9699-72
Mycobacterium tuberculosis, Early Secreted Antigenic Target Protein (ESAT6)
Description:
The ESAT-6 antigen from Mycobacterium tuberculosis is a dominant target for cell-mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models (2,5).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:3160
Optimal dilutions. to be determined by the researcher.

Hybridoma:
X63-Ag8.653. Female CF1 x Balb/c mice immunized i.p.

Culture Medium:
RPMI 1640 with 10% fetal calf serum.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k6F153Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
PPD from Mycobacterium tuberculosis (Tuberculin PPD, RT23, Statens Serum Institut) (1).
Purity:
Purified by Protein A/G affinity chromatography from culture supernatant.
Form
Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes a 10kD band from short term culture of M. tuberculosis identified as the protein ESAT-6 (Rv3875) in Western Blot. Binds to an epitope consisting of the residues EQQWNFAGIEAAA at positions 3-15 (4). Species Crossreactivity: Mycobacterium kansasii, Mycobacterium szulgai, and Mycobacterium marinum. Reacts with PPD, culture filtrate, cytosol and cell wall preparations from M. tuberculosis.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sorensen AL, et al., (1995) Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis. Infect Immun 63:1710-17. 2. Ravn P,et al., (1999) Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 179:637-645. 3. Klausen et al., (1994) Characterization of purified protein derivative of tuberculin by use of monoclonal antibodies: isolation of a delayed-type hypersensitivity reactive component from M. tuberculosis culture filtrate. Scand J Immunol 40:345-9. 4. Harboe M, et al., (1998) B-Cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis. Infect Immun 66:717-23. 5. Harboe M, et al., (1996) Evidence for occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG. Infect Immun 64:16-22.