Technical Data
M9699-74G
Mycobacterium tuberculosis 38kD (M. tuberculosis)
Description:
Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65kD heat shock protein or HSP65.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k10g208Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant M. tuberculosis protein cloned from virulent strain H37Rv with primers targeting the 38kD antigen.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in 0.01M PBS, pH 7.0. Reconstitute with sterile ddH2O to 1mg/ml.
Specificity:
Recognizes recombinant immunogen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.