Technical Data
M9750-15J
Mycoplasma pneumoniae
Description:
Mycoplasma pneumoniae is a very small bacterium, a member of the class Mollicutes, meaning soft skin. Along with the other members of this class (Acholeplasma, Anaeroplasma, Asteroleplasma, Spiroplasma, and Ureaplasma) Mycoplasma are characterized by their unusually small genome as well as their complete lack of a bacterial cell wall. M. pneumoniae is a common cause of mild pneumonia and usually affects people younger than 40. Various studies suggest that it causes 15 to 50% of all pneumonia in adults and an even higher percentage of pneumonia in school aged children. Beta lactam antibiotics such as penicillin and cycloserine are ineffective as they act specifically to disrupt the cell wall so alternative antibiotic therapies such as the use of polyenes may be required. People at highest risk of mycoplasma pneumonia infection include those living or working in crowded areas such as schools and homeless shelters, although many people who become infected have no identifiable risk factor. M. pneumoniae can be detected via serology, PCR or in cell culture assays.

Applications:
Suitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:10-1:50
ELISA: 1:20-1:200
Western Blot: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110D23Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Mycoplasma pneumoniae
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes multiple WB bands of Mycoplasma pneumoniae. Neg. with uninfected cells. No stablizing proteins added.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.