Technical Data
M9750-35C
Mycoplasma pneumoniae
Description:
Mycoplasma pneumoniae is a very small bacterium and a member of the class Mollicutes, meaning soft skin. Along with the other members of this class (Acholeplasma, Anaeroplasma, Asteroleplasma, Spiroplasma and Ureaplasma) Mycoplasma are characterized by their unusually small genome as well as their complete lack of a bacterial cell wall. M. pneumoniae is a common cause of mild pneumonia and usually affects people younger than 40. Various studies suggest that it causes 15 to 50% of all pneumonia in adults and an even higher percentage of pneumonia in school aged children. Beta lactam antibiotics such as penicillin and cycloserine are ineffective as they act specifically to disrupt the cell wall. So, alternative antibiotic therapies such as the use of polyenes may be required. People at highest risk of pneumonia infection from mycoplasma include those living or working in crowded areas such as schools and homeless shelters, although many people who become infected have no identifiable risk factor.

Applications:
Suitable for use in Immunofluorescence, ELISA, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10-1:50
Immunocytochemistry: 1:20-1:200
ELISA: 1:20-1:200
Western Blot: 1:20-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15E379Affinity Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Mycoplasma pneumoniae
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide,.
Specificity:
Recognizes a ~200kD antigen of Mycoplasma pneumoniae. Negative with uninfected cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.