Technical Data
MyD88, IN (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting: 1:500-1:1000. Whole cell lysate from Jurkat cells can be used as positive control and an ~35kD band can be detected.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Peptide corresponding to amino acids 233 to 248 of human MyD88 (5), which differ from those of mouse by two amino acids.
Purified IgG by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide. Stable for one year at 4 C.
Recognizes MyD88. An ~35kD band can be detected. It is human and mouse reactive.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muzio M,et al.,. Science 1997;278:1612-52. Adachi O, et al. Immunity 1998;9:143-50 3. Medzhitov R. et al Mol Cell 1998;2:253-8 4. Kawai T, et al. . Immunity 1999;11:115-22 5. Bonnert TP, et al FEBS Lett 1997;402:81-4