Technical Data
M9755-10D
MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)
Description:
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml. A protein of approximate molecular weight of 32kD is detected.
Optimal dilutions to be determined by the researcher.

Positive Control: Whole cell lysate from human spleen or Raw cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Peptide corresponding to amino acid residues 233-248 of human MyD88.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human MyD88.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hardiman G., et al., Oncogene 13 (11): 2467-2475 (1996). 2. Bonnert TP, et al., FEBS Lett 402: 81-84 (1997). 3. Burns K, et al.,. J Biol Chem 273 (20): 12203-12209 (1998). 4. Wesche H, et al., Immunity 7 (6): 837-847 (1997).