		Technical Data
M9755-10D	MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)

Description:
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml. A protein of approximate molecular weight of 32kD is detected.
Optimal dilutions to be determined by the researcher.

Positive Control: Whole cell lysate from human spleen or Raw cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Peptide corresponding to amino acid residues 233-248 of human MyD88.

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human MyD88.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hardiman G., et al., Oncogene 13 (11): 2467-2475 (1996). 2. Bonnert TP, et al., FEBS Lett 402: 81-84 (1997). 3. Burns K, et al.,. J Biol Chem 273 (20): 12203-12209 (1998). 4. Wesche H, et al., Immunity 7 (6): 837-847 (1997).