Technical Data
M9755-10E
MyD88 (Myeloid Differentiation Marker 88, Myeloid Differentiation Primary Response Gene, Myeloid Differentiation Primary Response Protein MyD88)
Description:
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adaptor molecule in the IL-1 signaling pathway. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and the dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor, which are members of the Toll/IL-1R family of receptors.

Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adaptor protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. The MyD88 gene is expressed in many tissues.

Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
MOLT-4 and Jurkat cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.25 mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of the human MyD88 protein.
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes human MyD88 protein at ~33 kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonnert TP, et al. FEBS Lett 402:81-84, 1997. 2. Burns K, et al. J Biol Chem 273:12203-12209, 1998. 3. Medzhitov R, et al. Mol Cell 2:253-258, 1998. 4. Muzio M, et al. Science 278:1612-1615, 1997. 5. Wesche H, et al. Immunity 7:837-847, 1997.6. Kawai T, et al. Immunity 11:115-122, 1999.