Technical Data
Myelin is dielectric (electrically insulating) material that forms a layer, the myelin sheath. Usually, myelin surrounds only the axon of a neuron. It is essential for proper functioning of the nervous system. Myelin is an outgrowth glial cell: Schwann cells supply the myelin for peripheral neurons, whereas oligodendrocytes supply it to those of the central nervous system. Myelin is considered a defining characteristic of the (gnathostome) vertebrates, but it has also arisen by parallel evolution in some invertebrates. Myelin was discovered in 1854 by Rudolf Virchow.

Myelin made by different cell types varies in chemical composition and configuration, but performs the same insulating function. Myelinated axons are white in appearance, hence the "white matter" of the brain. Myelin is composed of about 80% lipid and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG), and Proteolipid protein (PLP). Myelin is made up primarily of a glycolipid called galactocerebroside. The intertwining of the hydrocarbon chains of sphingomyelin serve to strengthen the myelin sheath.

The main purpose of a myelin layer (or sheath) is an increase in the speed at which impulses propagate along the myelinated fiber. Along unmyelinated fibers, impulses move continuously as waves, but, in myelinated fibers, they hop or "propagate by saltation." Myelin increases electrical resistance across the cell membrane by a factor of 5,000 and decreases capacitance by a factor of 50.Thus, myelination helps prevent the electrical current from leaving the axon. When a peripheral fiber is severed, the myelin sheath provides a track along which regrowth can occur. Unmyelinated fibers and myelinated axons of the mammalian central nervous system do not regenerate.

Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and paraffin sections, no proteolytic treatment required).

Recommended Dilution:
Immunohistochemistry: 1:10, 1 hr at RT.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20°CBlue IceHumanMouse
Not determined
Human myelin isolated from peripheral nervous system.
Supplied as a liquid.
Recognizes only myelin in the peripheral nervous system (PNS). In frozen sections it also reacts with myelin in the central nervous system (CNS). Is useful for studying myelination and demyelination processes in the PNS, and remyelination processes in the CNS as can be observed after trauma in multiple sclerosis. Reacts with a 43kD protein in immunoblot. Recommended for Peripheral nerve. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kros JM et al. Ultrastructural and immunohistochemical segregation of gemistocytic subsets. Human Path 22, 33-40 (1991)
General References:
Virchow R (1854) Über das ausgebreitete Vorkommen einer dem Nervenmark analogen Substanz in den tierischen Geweben. Virchows Arch. Pathol. Anat. 6:562-572.
Ledeen R.W., Chakraborty G., "Cytokines, Signal Transduction, and Inflammatory Demyelination: Review and Hypothesis" Neurochemical Research, Volume 23, Number 3, March 1998, pp. 277-289(13)
FuturePundit January 20, 2004
Krämer-Albers at al., 2006
Matalon et al., 2006
Tkachev et al., 2007