Technical Data
M9757-50
Myelin Associated Glycoprotein (MAG)
Description:
Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100kD, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Useful for myelinogenesis and multiple sclerosis studies. Other applications not tested.

Recommended Dilutions:
Western Blot: 20ug/ml, non-reducing conditions only
Immunohistochemistry: Frozen and Paraffin
Immunocytochemistry: 5-10ug/ml, methanol fixation required. 1. Oligodendrocytes are cultivated on slides and fixed with methanol for 10 minutes at-20C in the middle log-phase.
2. After blocking the nonspecific binding sites with 5% FCS the cells are incubated with the properly diluted M9757-50 in a humid chamber for one hour at RT.
3. Afterwards cells are washed and incubated with anti-Mouse IgG-FITC. The slides are washed and covered appropriately
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat hippocampus tissue, rat brain lysate, PC12 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q144Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceChickenMouse
Concentration:
~1mg/ml
Immunogen:
L2 epitope carrying glycoprotein extract of 12 day old chicken brain.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5. No preservative added.
Specificity:
Recognizes chicken Myelin Associated Glycoprotein at ~100kD. Species Crossreactivity: human, rat, mouse, bovine and frog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Trillo-Pazos, G., et al. (2004). Neuropathol. Appl. Neurobiol. 30(2):136-147. 2. Goolsby, J., et al. (2003). PNAS. 100:14926- 14931. 3. Ellezam, B., et al. (2003). Neurobiol Disease. 12:1-10. 4. Pang, Y., et al. (2000). J. Neurosci. Res. 62(4):510-520. 5. Collins, BE., et al. (1997). J Biol Chem. 272(27):16889-16895.