Technical Data
M9757-50C
MAG (Myelin Associated Glycoprotein, GMA, S-MAG, Siglec 4a, SIGLEC4A)
Description:
Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100kD, is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron. Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury.

Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20
Western Blot: 1:500-1:5000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9H477Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues near the N-terminus of human MAG.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human MAG at ~100kD. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Barton, D.E., et al., Genomics 1(2): 107-112 (1987). 2. Liu, B.P., et al., Science 297(5584): 1190-1193 (2002). 3. Marta, C.B., et al., Neuron Glia Biol. 1(1): 35-46 (2004). 4. Tang, S., et al., Mol. Cell. Neurosci. 9(5-6): 333-346 (1997).