Technical Data
M9758-01
Myelin Basic Protein, aa130-136 (MBP)
Description:
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles. MBP serves as a marker for oligodendrocytes and Schwann cells. MBP is also a marker for malignant schwannomas and appears to play a significant role in the etiology of multiple sclerosis and experimental autoimmune encephalomyelitis.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and quantification of myelin basic protein. Other applications have not been tested.

Recommended Dilutions:
ELISA: 10-20ug/ml
Western Blot: 1-2ug/ml
Immunohistochemistry (Frozen, parrafin): 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b1.B.645Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified human myelin basic protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes residues 130-136 in human myelin basic protein (21.5kD and 18.5kD molecular forms). Species Crossreactivity: primate, rabbit, sheep, goat, rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.