Technical Data
M9758-01E
Myelin Basic Protein (MBP, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MGC99675, Myelin Deficient, MLD, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, Shiverer, SHI, SP)
Description:
The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Paraffin): 1:50-1:100 Incubate for 30 minutes at RT using ABC detection method.
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Human myelin basic protein isolated from normal human brain. Cellular Localization: Cytoplasmic
Purity:
Serum
Form
Supplied as a liquid with sodium azide.
Specificity:
Recognizes human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.