Technical Data
Myelin Basic Protein (MBP, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MGC99675, Myelin Deficient, MLD, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, Shiverer, SHI, SP)
Cellular Localization: Cytoplasmic side of myelin

Suitable for use in AF, RID, Immunofluorescence, ELISA, Western Blot and Immunohistochemistry (Fr). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20CBlue IceBovineRat
As reported
Full length protein (Cow). Epitope: Amino acids 82-87.
Tissue culture supernatant
0.1% Sodium Azide; Tissue Culture Supernatant
The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Species Crossreactivity: Crossreacts with Human, bovine, Guinea pig, Mouse, Rabbit, Rat and Sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Groome NP et al. Region-specific immunoassays for human myelin basic protein. J Neuroimmunol 12:253-64 (1986). Glynn P et al. Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments. J Neurochem 48:752-9 (1987).