Technical Data
Myelin Basic Protein (MBP, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MGC99675, Myelin Deficient, MLD, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, Shiverer, SHI, SP)
Myelin basic protein (MBP) is an intracellular protein with highly flexible structure found in myelin of central and peripheral nervous system (CNS, PNS). The classic group of MBP isoforms (isoforms 4-14) with PLP is the major protein components of the myelin membrane in CNS and functions in its formation and stabilization. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) has been implicated in early developing brain long before myelination, possible components of transcriptional complexes, and possible role in T-cells and neural cells signaling pathways. MBP is often post-translationally modified in various ways, which includes citrullination, N-terminal acylation, deamidation, and phosphorylation (1). Surface charge reduction of citrullinated and/or methylated MBP has been linked in weakening the myelin membrane, which can lead to demyelinating diseases such as chronical multiple sclerosis (MS) (2).

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:60
Western Blot: 1:1000-1:5000
Immunoprecipitation: 1:20
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues located between amino acids 150 to 185 of human MBP.
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human MBP. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yong Ma et al J. Biochem, 122(4):810-818 1997 2. Moscarello, MA et al. Neurochem Res. 32(2):251