Technical Data
M9758-01N
Myelin Basic Protein (MBP, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MGC99675, Myelin Deficient, MLD, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, Shiverer, SHI, SP)
Description:
Myelin basic protein (MBP) is an intracellular protein with highly flexible structure found in myelin of central and peripheral nervous system (CNS, PNS). The classic group of MBP isoforms (isoforms 4-14) with PLP is the major protein components of the myelin membrane in CNS and functions in its formation and stabilization. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) has been implicated in early developing brain long before myelination, possible components of transcriptional complexes, and possible role in T-cells and neural cells signaling pathways. MBP is often post-translationally modified in various ways, which includes citrullination, N-terminal acylation, deamidation, and phosphorylation (1). Surface charge reduction of citrullinated and/or methylated MBP has been linked in weakening the myelin membrane, which can lead to demyelinating diseases such as chronical multiple sclerosis (MS) (2).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:60
Western Blot: 1:1000-1:5000
Immunoprecipitation: 1:20
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabIgG9H478Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues located between amino acids 150 to 185 of human MBP.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Specificity:
Recognizes human MBP. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yong Ma et al J. Biochem, 122(4):810-818 1997 2. Moscarello, MA et al. Neurochem Res. 32(2):251