Technical Data
M9758-01S
MBP (Myelin Basic Protein, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675)
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Technical Data |
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M9758-01S |
MBP (Myelin Basic Protein, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675) |
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Description: Myelin sheaths, comprised of oligodendrocytes and Schwann cells, are critical to action potential propagation in the axon of a neuron. Myelin basic protein (MBP) (~18.5kD) is one of the most abundant membrane proteins in the central nervous system. Through interaction with proteolipid protein (PLP), protein zero (P0) and other membrane- associated proteins, MBP is responsible for the compaction and stability of the myelin sheath layers. Demyelination of axons occurs in autoimmune diseases such as multiple sclerosis (MS) and usually results in impairment of motor and sensory functions. MBP is studied to be a major focus in MS progression. Current research finds that MBP undergoes deimination that converts arginines to citrullines, ultimately causing synergistic neurodegeneration in chronic MS patients. MBP, along with PLP and myelin oligodendrocyte glycoprotein (MOG), is also widely used as an immunodominant epitope to induce experimental autoimmune encephalomyelitis (EAE), an animal model for MS. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:1000 Immunohistochemistry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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