Technical Data
M9758-03B-Biotin
MBP, phosphorylated (Tyr203) (Myelin Basic Protein, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein) (Biotin)
Description:
Myelin basic protein (MBP) is a protein believed to be important in the process of myelination of nerves in the central nervous system (CNS). The pool of MBP in the central nervous system is very diverse, with several splice variants being expressed and a large number of post-translational modifications on the protein, which include phosphorylation, methylation, deamidation and citrullination.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr203 of human MBP (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity:
Recognizes human MBP when phosphorylated at Tyr203.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kawamura,K., J. Immunol. 181(5), 3202-3211 (2008). 2.Majava,V., BMC Struct. Biol. 8, 10 (2008). 3.Boylan,K.B.,Genomics 6(1), 16-22 (1990).