Technical Data
M9758-03G
Myelin Basic Protein, aa119-131 (MBP)
Description:
Myelin basic protein (MBP) is a single-chain, flexible polypeptide of about 18.5kD existing in its natural environment as an extrinsic and loosely bound component of the cytoplasmic portion of the myelin membrane sandwich. Ultrastructural immunocytochemistry with anti-myelin basic protein has shown that MBP is localized in the compact myelin sheath. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes or any other cytoplasmic organelles (3). MBP serves as a marker for oligodendrocytes and Schwann cells (3). MBP is also a marker for malignant schwannomas (4) and appears to play a significant role in the etiology of multiple sclerosis (57) and experimental autoimmune encephalomyelitis (8).

Applications:
Suitable for use in Immunocytochemistry, ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunocytochemistry: Frozen sections

Western Blot: 10ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q288Supernatant
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IceBovineMouse
Concentration:
~10-50ug/ml
Immunogen:
Bovine myelin basic protein
Purity:
Tissue culture supernatant
Form
Supplied as a liquid in 0.2M Tris/HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
Specificity:
Reacts with MBP from human, bovine, rabbit, rat, sheep and guinea pig. The epitope appears to be located in the region of residues 119-131.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Day, E.D. and Potter, N.T., J. Neuroimmunol. 10: 289 (1986). 2. Hashim, G.A., Immunol. Rev. 39: 60 (1978). 3. Golo, M., Histochemistry 87: 201 (1987). 4. Mogollon, R., et al., Cancer 53: 1190 (1984). 5. Whitaker, J.N., Ann. N.Y. Acad. Sci. 436: 140 (1984). 6. Eidinoff, H., Med. Hypotheses 26: 103 (1988). 7. tar Meulen, V., Ann. N.Y. Acad. Sci. 540: 202 (1988). 8. Kerlero de Rosbo, N., et al., J. Neuroimmunol. 9: 349 (1985). 9. Sires, L.R., et al., Science 214: 87 (1981). 10. Groome, N.P., et al., Neurochemistry Int. 7: 309-317 (1985). 11. Martenson, R.E., in Experimental Allergic Encephalomyelitis. A useful model for Multiple Sclerosis (Alvord, Kies and Suckling eds) pp 511521. Alan Liss N.Y. (1984). 12. Groome, N.P., et al., J. Neuroimmunol. 12: 253-264 (1988). 13. Cai, Z., et al., Brain Research 898: 126-135 (2001).