Technical Data
M9758-03M
Myelin Basic Protein, aa36-50 (MBP)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Western Blot: Reported
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized outbred rats were fused with cells of the mouse NSO myeloma cell line.

Recommended Secondary Reagents:
F(ab')2 rabbit anti-rat IgG HRP

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3H2748Supernatant
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceBovineRat
Concentration:
Not determined.
Immunogen:
Bovine myelin basic protein aa36-50
Purity:
Tissue culture supernatant
Form
Supplied as a liquid in 0.1% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes myelin basic protein, recognizing a synthetic peptide corresponding to amino acids 36-50, as strongly as intact human MBP. Species Crossreactivity: Most mammalian species, but weak with rabbit. Not tested on mouse or rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264.
2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759.
3. Groome, N.P. et al. (1988). New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein. J. Neuroimmunol. 19: 305-315.