Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: Frozen
Western Blot: Reported
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized outbred rats were fused with cells of the mouse NSO myeloma cell line.
Recommended Secondary Reagents:
F(ab')2 rabbit anti-rat IgG HRP
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2b | 3H2748 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1ml | | 4°C (-20°C Glycerol) | Blue Ice | Bovine | Rat |
|
| Concentration: |
| Not determined. |
| Immunogen: |
| Bovine myelin basic protein aa36-50 |
| Purity: |
| Tissue culture supernatant |
| Form |
| Supplied as a liquid in 0.1% sodium azide before the addition of glycerol to 40%. |
| Specificity: |
| Recognizes myelin basic protein, recognizing a synthetic peptide corresponding to amino acids 36-50, as strongly as intact human MBP. Species Crossreactivity: Most mammalian species, but weak with rabbit. Not tested on mouse or rat. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264.
2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759.
3. Groome, N.P. et al. (1988). New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein. J. Neuroimmunol. 19: 305-315.
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