Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Western Blot: Reported
Optimal dilutions to be determined by the researcher.
Spleen cells from immunized outbred rats were fused with cells of the mouse NSO myeloma cell line.
Recommended Secondary Reagents:
F(ab')2 rabbit anti-rat IgG HRP
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|1ml||4°C (-20°C Glycerol)||Blue Ice||Bovine||Rat|
| Not determined.|
|Bovine myelin basic protein aa36-50|
|Tissue culture supernatant|
|Supplied as a liquid in 0.09% sodium azide before the addition of glycerol to 40%.|
|Recognizes myelin basic protein, recognizing a synthetic peptide corresponding to amino acids 36-50, as strongly as intact human MBP. Species Crossreactivity: Most mammalian species, but weak with rabbit. Not tested on mouse or rat.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264.|
2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759.
3. Groome, N.P. et al. (1988). New monoclonal antibodies reactive with defined sequential epitopes in human myelin basic protein. J. Neuroimmunol. 19: 305-315.