Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA and RIA. Other applications have not been tested.
Recommended Dilution:
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents:
Goat Anti Rat IgG: HRP (mouse absorbed)
Fusion Partners:
Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG | 3H2750 | Supernatant |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 2ml | | 4°C (-20°C Glycerol) | Blue Ice | Bovine | Rat |
|
| Concentration: |
| As reported |
| Immunogen: |
| Bovine MBP. |
| Purity: |
| Tissue culture supernatant. |
| Form |
| Supplied as a liquid with 0.1% sodium azide. |
| Specificity: |
| Reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 has been reported as being suitable for use in Western blotting. Species Crossreactivity: Reacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3). |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264. 2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759. 3. Hruby, S. et al . (1987). Monoclonal antibodies reactive with myelin basic protein. Molecular Immunology. 24(12): 1359 - 1364
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