Technical Data
M9758-03P
Myelin Basic Protein, aa82-87 (MBP)
Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA and RIA. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
Goat Anti Rat IgG: HRP (mouse absorbed)

Fusion Partners:
Spleen cells from an immunized outbred rat were fused with cells of the mouse NSO myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG3H2750Supernatant
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IceBovineRat
Concentration:
As reported
Immunogen:
Bovine MBP.
Purity:
Tissue culture supernatant.
Form
Supplied as a liquid with 0.1% sodium azide.
Specificity:
Reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Clone 3H2750 has been reported as being suitable for use in Western blotting. Species Crossreactivity: Reacts widely with mammalian species including human, bovine, sheep, rabbit (3), mouse, rat, guinea pig and chicken (3).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Groome, N.P. et al. (1986). Region specific immunoassays for myelin basic protein. J. Neuroimmunol. 12: 253-264. 2. Glynn, P. et al. (1987). Basic protein dissociating from myelin membranes at physiological pH is cleaved into three major fragments. J. Neurochem. 48: 752-759. 3. Hruby, S. et al . (1987). Monoclonal antibodies reactive with myelin basic protein. Molecular Immunology. 24(12): 1359 - 1364