Description:
Fusion Partners: Spleen cells from immunized A/J mice were fused with cells of the NSO mouse myeloma cell line.
Applications:
Immunohistochemistry: (frozen)- 1:100
ELISA
Western Blot
Optimal working dilutions to be determined by researcher.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 3H2751 | Ascites |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 250ul | | -20°C | Blue Ice | Bovine | Mouse |
|
| Concentration: |
| As reported |
| Immunogen: |
| Bovine Myelin Basic protein (MBP) (45-91) / Ovalbumin Conjugate. |
| Purity: |
| Ascites. |
| Form |
| Ascites fluid - liquid in with 0.1% sodium azide. |
| Specificity: |
| It appears to react with synthetic peptide 82-91 and human and bovine 45-91 but not with whole MBP molecules (Martensen numbering). This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP.The clone was selected using synthetic human MBP peptide 82-91 for screening (1). Since this work was done a seqence error has been described in the human MBP seqence in this region (3). Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, rather than the correct human 82-91. In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published (1).The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Groome, N.P., Harland, J. and Dawkes, A. (1985). Preparation and characterisation of monoclonal antibodies to myelin basic protein and its peptides. Neurochemistry International. 7: 309-317.2. Martensen, R.W. (1984) in Experimental Alle
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