Technical Data
M9758-03Q
Myelin Basic Protein, aa82-91 (MBP)
Description:
Fusion Partners: Spleen cells from immunized A/J mice were fused with cells of the NSO mouse myeloma cell line.

Applications:
Immunohistochemistry: (frozen)- 1:100
ELISA
Western Blot
Optimal working dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG2a3H2751Ascites
SizeStorageShippingSourceHost
250ul-20CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Bovine Myelin Basic protein (MBP) (45-91) / Ovalbumin Conjugate.
Purity:
Ascites.
Form
Ascites fluid - liquid in with 0.1% sodium azide.
Specificity:
It appears to react with synthetic peptide 82-91 and human and bovine 45-91 but not with whole MBP molecules (Martensen numbering). This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP.The clone was selected using synthetic human MBP peptide 82-91 for screening (1). Since this work was done a seqence error has been described in the human MBP seqence in this region (3). Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, rather than the correct human 82-91. In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published (1).The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Groome, N.P., Harland, J. and Dawkes, A. (1985). Preparation and characterisation of monoclonal antibodies to myelin basic protein and its peptides. Neurochemistry International. 7: 309-317.2. Martensen, R.W. (1984) in Experimental Alle