Technical Data
M9758-03X
Myelin Basic Protein (MBP, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, MGC99675)
Description:
Myelin basic protein (MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (Human, rat): 1ug/ml
Immunohistochemistry (Paraffin) (Human, rat, mouse): 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH20. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to a sequence from the C-terminal of the human Myelin Basic Protein. Species Sequence Homology: rat, mouse; 100%
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity:
Recognizes the human Myelin Basic Protein. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marty MC., et al. Proc Natl Acad Sci USA 2002 Jun 25; 99(13):8856-61. 2. Streicher R., Stoffel, W. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10. 3. McLaurin, JA., et al. J Neurol Sci. 1995 Feb; 128(2):205-11. 4. Lester, A., et al. Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5. 5. Tranquill., LR., et al. J Neurosci Res. 1996 Sep 15; 45(6):820-8.